The Roughriders have had 12 players signed to professional contracts by 13 different organizations.
| player | year acquired | organization | Roughrider years |
| Lee DeLoach | 1989 | Los Angeles (NL, MLB) | 1987-88, '91-99 |
| Bob Rivell | 1990 | San Diego (MLB) | 1995 |
| George Lovett | 1996 | Clarksville (IND) | 1992-96, ‘98-2002 |
| 2001 | Tennessee (IND) | ||
| 2002 | Yuma (IND) | ||
| BJ Amorelli | 1996 | Clarksville (IND) | 1990-95, ‘97-98, 2000-05 |
| J.R. Shalick | 1998 | Baltimore (MLB) | 1997 |
| George West | 1998 | Milwaukee (MLB) | 1995 |
| Guy Lynam | 2001 | Florida (MLB) | 2001 |
| 2003 | Colorado (MLB) | ||
| 2005 | Camden (IND) | ||
| Jonathan George* | 2002 | Cincinnati (MLB) | 2000-01 |
| 2006 | Colorado (MLB) | ||
| Mark Michael | 2003 | St. Louis (MLB) | 2002 |
| Dan McKenna | 2003 | Milwaukee (MLB) | 2000-02 |
| 2005 | Camden (IND) | ||
| Josh Laxton | 2005 | Camden (IND) | 1997-98, 2000 |
| Steve Wyland | 2007 | Brockton (IND) | 2004, '06-present |
| 2008 | Atlantic City (IND) |
*still active